DEV025627 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Widespread apoptosis and angiogenesis defects in Cts8-overexpressing embryos. (A) TUNEL staining on sections of wild-type and transgenic (CMV-Cre.tg^Cts8) embryos. Large patches of apoptotic cells (arrowheads) are seen in neuroectoderm and mesenchyme, in particular surrounding somites (arrowhead). The boxed area is shown in magnification in the adjacent panel. (B) Pecam/CD31 immunostaining shows lack of a mature vascular plexus in tg^Cts8 (Sox2-Cre.tg^Cts8) embryos. The dorsal aorta is only faintly stained and severely dilated in transgenic embryos (arrows). Scale bars: 200 µm and 100 µm in A; 500 µm in B.