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Figure 3


Fig. 3. Transgenic mouse models for inducible expression of Cts7. (A) General structure of the construct used. (B) β-Galactosidase staining proves strong, ubiquitous transgene activity before activation with Cre recombinase. (C) Northern blot demonstrating strong Cts7 expression in the embryo after ubiquitous activation with Cre. (D) E12.5 wild-type and tgCts7 (Sox2-Cre.tgCts7) placentas hybridized with Cts7, Tpbpa and Prl3b1. (E) Northern blot analysis and relative expression levels of marker genes in wild-type and transgenic (Sox2-Cre.tgCts7) placentas. *P<0.05. Scale bars: 500 µm in B; 1 mm in D.





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