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Figure 5


Fig. 5. Cts8 overexpression promotes giant cell differentiation. (A) Northern blot hybridization with Cts8 on wild-type and tgCts8 placentas showing approximately equal amounts of endogenous (endo. Cts8) and transgenic (tg Cts8) Cts8 mRNA. The transgenic product is a bicistronic Cts8-GFP RNA. (B) Marker expression analysis of E9.5 placentas after ubiquitous transgene activation (Sox2-Cre.tgCts8). Note the particularly strong transgene expression in extra-embryonic mesoderm and parietal endoderm. No striking phenotype is observed at this stage. (C) Prl3b1 expression on E16.5 placentas after trophoblast-specific Cts8-activation by mating to Tpbp-Cre mice. The giant cell/spongiotrophoblast layer is enlarged. (D) Trophoblast tissue dissected from E9.5 control and ubiquitously induced Sox2-Cre.tgCts8 placentas after 2 days culture stained with DAPI. Fewer diploid but more and bigger giant cells are observed. (E) Quantification of nuclear sizes. No giant cell larger than 1200 µm2 was found in controls. Scale bars: 500 µm in B; 1 mm in C; 1 mm in D.





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