|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Cell lineage tracing of Isl1+ cardiac progenitors and lineage
contribution to the adult heart. Lineage tracing utilizing the
Isl1-IRES-Cre mouse crossed with the indicator R26R line to mark all
cells that once expressed Isl1 during development. The boxes represent
magnifications of different regions of the coronary tree (black boxes) and of
the aorta/pulmonary artery (red boxes), where β-gal-positive cells have
been detected. (A) Contribution of Isl1+ progenitors (blue)
to the coronary vasculature, the valves and the pulmonary artery/aorta. The
contribution of Isl1+ cells to the endothelial (purple) and smooth
muscle (yellow) cell layers is limited to the proximal area of the great
vessels and progressively declines from the proximal to the distal parts of
the coronary tree. β-gal+ cells were also detected in
connective tissue structures of the aortic and pulmonary leaflets, indicating
that components of the conotruncal cushions, which have an endocardial origin,
are derived from Isl1+ progenitors. (B) Contribution of
Isl1+ progenitors (blue) to the atrial/ventricular myocardium
(red), the conduction system (white) and cardiac ganglia (green). In the
conduction system, most genetically marked cells were detected in the
sino-atrial nodal (SAN) region. AS, atrial septum; AVN, atrioventricular node;
CAs, coronary arteries; LA, left atrium; LCA, left coronary artery; LV, left
ventricle; RA, right atrium; RCA, right coronary artery; RV, right ventricle;
VS, ventricular septum.
|
