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Files in this Data Supplement:
Fig. S1. RT-PCR analysis of CG8062 expression. (A) Primers specific for CG8062 were designed to amplify a 634 bp cDNA, spanning exons 3 and 4. RNA was extracted from 0- to 15-hour-old embryos. cDNAs derived from out 1, out 2, out 4, out 5 and the wild type amplified a 634 bp cDNA fragment of CG8062 (lanes 2-6). The CG8062 transcript was not detected in KG07784 (lane 7). The 716 bp fragment amplified from genomic DNA containing intron 3 is shown as a control (lane 8). (B) Primers specific for ribosomal protein 49 were used as a positive control. All samples amplified a 95 bp cDNA fragment. The 157 bp genomic DNA fragment is also shown (lane 8).
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