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Fig. 2. Expression of Corin protein coincides with Corin transcript
accumulation. (A) Schematic representation of Corin mRNA
and protein. The mRNA of Corin (shown in red) comprises 22 exons
encoding a type-II transmembrane serine protease. The single-pass
transmembrane domain (TM) of Corin resides in close proximity to the
N-terminus, and the large extracellular portion includes two frizzled-like
cysteine-rich motifs (designated Frizzled 1 and 2), eight LDL receptor repeats
(LDLR1-8), a macrophage scavenger receptor-like domain (SRCR) and a catalytic
domain of trypsin-like serine protease at the C-terminus. The active site
residues of the catalytic triad (H, D and S) are shown. The regions
corresponding to the RNA probe and the fragment of Corin protein used to
immunize rabbits are indicated. (B-F) Immonuhistochemical detection of
Corin (green) in DP at E17.5 (B), P0 (C), P3 (D), P6 (E) and P11 (F). Red
stain highlights nuclei. Occasional staining was observed outside the DP
(white arrowheads) but this was also detected in controls in which only the
secondary antibody was used (data not shown and supplementary material Fig.
S2). In E and F, higher magnification of a bulb region is shown (upper right)
to reveal that the majority of the protein is localized at the periphery of DP
cells.
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