|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Analysis of the RNA levels of Corin, agouti,
Mc1r, Pomc1, Atrn and Mgrn1 throughout the
anagen phase of the hair cycle. (A-F) The relative RNA levels
determined by real-time PCR (y-axis) of Corin (A),
agouti (B), Mc1r (C), Pomc1 (D), Atrn (E)
and Mgrn1 (F) are shown from birth to P9 (x-axis) for
Corin+/+ (black) and Corin-/- (pink)
mice. The RNA levels of all genes tested were normalized to the same units,
but different scales used in A,E,F vs. B,C vs. D. Thus the level of
Atrn mRNA is about fivefold higher than the RNA levels of
agouti at its peak (B,E), whereas Pomc1 levels are similar to those
of agouti at its baseline levels from P7 onwards (B,D). Data are
means ± s.d. (G) In situ hybridization of agouti in P5
skin. Asterisks indicate hair follicle bulbs with agouti expression
in the DP and eumelanin deposition. Note that in the absence of
Corin, eumelanin deposition has not been detected in the bulb region
surrounding agouti-expressing DPs. In the right upper corner, higher
magnification of the region enclosed by the red square is shown.
|
