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Fig. 6. The Xist locus on XpA fails to establish the
repressive histone modification. Chromatin of the visceral endoderm was
used for ChIP assays with the antibodies against the respective histone
modifications. The immunoprecipitated chromatin was quantified by real-time
PCR using Xist1F and Xist101R as primers, which amplify the first 101 bp
sequence of Xist exon 1. The value for each modification, which is shown as
relative abundance of the chromatin immunoprecipitated by each antibody
compared with the input, was obtained from two or three independent
experiments. Values are means ± s.d.