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Fig. 4. Comparative expression analysis of Notch signaling components and
FoxE3 in X. laevis embryos, showing that Notch2 and
FoxE3 are expressed in PLE, while Delta1 and Delta2
are expressed in presumptive retina. Expression of Notch2
(A-B'), Delta1 (C-E'), Delta2
(F-H'), and FoxE3 (I-K') was detected by in
situ hybridization from neural plate stages to early tailbud stages. Regions
circled with black dotted lines in C and F are the approximate presumptive
retina fields, where neither Delta1 nor Delta2 is expressed.
Arrows in B,B', D-E', G-H' and J-K' indicate
expression of Notch2, Delta1, Delta2 and
FoxE3, respectively. The black arrowhead in C indicates
Delta1 expression in the anterior neural ridge, and white arrowheads
in A and I indicate Notch2 and FoxE3 expression in the
pre-placodal ectoderm, respectively. White lines in E,H,K indicate the planes
of transverse eye sections shown in E',H',K'.
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