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Figure 4


Fig. 4. Identification of potential protein isoforms of FILIA in mice. (A) Predicted primary structure of 48 kDa (upper) and 38 kDa (lower) isoforms with 440 and 346 amino acids, respectively. N-terminal peptide immunogen common to both isoforms is underlined (black) and regions (amino acids 120-350 and 120-340, respectively) containing a 23 amino acid repeat are indicated by a dashed line (black). Sequences unique to each are bold and italicized. The ~50 kDa band (Fig. 2A) containing the presumptive 38 kDa isoform was digested with trypsin and microscale mass spectrometry identified peptides (red) common to both isoforms. (B) FILIA contains a novel, 23 amino acid tandem repeat (120-350 amino acids) defined by RADAR (Heger and Holm, 2000). Amino acids are color-coded: aliphatic, red; uncharged polar, green; acidic, blue; basic, yellow; neutral, magenta. (C) Fluorography of in vitro translation with [35S]methionine of FILIA cDNA encoding the 1.6 (lane 1) and 1.2 (lane 2) kb isoforms of FILIA protein after separation by SDS-PAGE. Molecular masses (kDa) are indicated on the left.





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