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Fig. 7. FILIA interacts with MATER. (A) Immunoprecipitation of MATER
with antibodies to FILIA. Ovulated eggs (
120) from wild-type (lanes
1,2,4) or Matertm/tm (lane 3) mice were lysed (0.5% Triton
X100 in PBS) and either analyzed directly (lane 1) or immunoprecipitated with
peptide-purified sheep antibodies to FILIA (lanes 2,3) or pre-immune sera
(lane 4). Proteins were resolved by SDS-PAGE, transferred to nitrocellulose,
incubated with peptide-purified rabbit antibodies to MATER and visualized with
chemiluminescence. (B) Expression vectors containing full-length MATER
(MYC-tagged) and FILIA were co-transfected into 293T cells (lanes 2,4).
Controls (lanes 1,3) were co-transfected with the MYC vector lacking MATER and
the FILIA expression vector. Whole cell lysates were analyzed before (left
panel) or after (right panel) immunoprecipitation with antibodies to MYC.
Proteins were resolved on SDS-PAGE gels, transferred to nitrocellulose and
probed with antibodies to MATER (left) or FILIA (right panel). Molecular
masses (kDa) are indicated at the left for both A and B.
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