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Fig. 8. Plasticity of FILIA and MATER localization during preimplantation
development. (A) Zona-free two-cell mouse embryos were incubated
with [(+) Ca2+ (a-d)] or without [(-) Ca2+ (e-h)]
calcium to induce disaggregation. After fixation and permeabilization, embryos
were stained with antibodies specific to FILIA (a,e), MATER (b,f) and
phalloidin, which binds to F-actin (c,g), before imaging by confocal (a-c,e-g)
or differential interference contrast (DIC) microscopy (d,h). (B) Same
as A except individual and clumps of blastomeres were isolated from single
zona-free morulae in the absence of calcium for localization of FILIA (a),
MATER, (b), actin (c) or visualization by DIC microscopy (d). (C) Cell
cleavage of blastomeres can be parallel or orthogonal to the axis of polarity.
If parallel, the daughter cells are equivalent; if orthogonal, the `outer'
cells seemingly contain the FILIA-MATER complex and `inner' cells do not. The
image is from Fig. 6.
(D) The ICM of early two (a-c; d-f) blastocysts was isolated by
immunosurgery and imaged as in A with antibodies to FILIA (a,d) and MATER
(b,e) or visualized by DIC (c,f). Normal blastocysts served as positive
imaging controls (data not shown). Scale bars: 20 µm in A,B,D.
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