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Fig. 2. HoxCP and tsh genes repress optix expression
in the trunk. (A-C) optix expression (green) in wild type,
stage 6 (A), 11 (B) and 14 (C). Arrows, early expression domain in the labrum;
arrowheads, patches of optix-expressing cells in maxillary and labial
segments. Clbr, Clypeolabrum; Spog, Supra oesophagial ganglia.
(D,E) Expression of optix in HoxCP and
tsh- HoxCP embryos at retraction. Elav (red) highlights
the ventral side of the embryo. White arrows indicate ectopic patches of
optix (green) in the trunk. The expression domain of optix
in the ventral labial segment is not visible in this section (D, asterisk, see
E and F). (F) tsh- HoxCP embryo showing
optix compared with engrailed (red) at stage 12. The
patterns are complementary, indicating that optix ectopic stripes are
located in the anterior region of each segment. There is an ectopic patch of
optix in the labial (Lb) and ninth abdominal segments (A9). B, D and
E are ventral views; A, C and F are lateral views.