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Figure 2


Fig. 2. HoxCP and tsh genes repress optix expression in the trunk. (A-C) optix expression (green) in wild type, stage 6 (A), 11 (B) and 14 (C). Arrows, early expression domain in the labrum; arrowheads, patches of optix-expressing cells in maxillary and labial segments. Clbr, Clypeolabrum; Spog, Supra oesophagial ganglia. (D,E) Expression of optix in HoxCP and tsh- HoxCP embryos at retraction. Elav (red) highlights the ventral side of the embryo. White arrows indicate ectopic patches of optix (green) in the trunk. The expression domain of optix in the ventral labial segment is not visible in this section (D, asterisk, see E and F). (F) tsh- HoxCP embryo showing optix compared with engrailed (red) at stage 12. The patterns are complementary, indicating that optix ectopic stripes are located in the anterior region of each segment. There is an ectopic patch of optix in the labial (Lb) and ninth abdominal segments (A9). B, D and E are ventral views; A, C and F are lateral views.





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