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Fig. 3. optix is required for the development of the labrum and mouth hooks during embryogenesis. (A) Genomic region of optix showing the insertions and the probes (red lines) used for the RNAi experiments. (B-G) Cuticle preparations of wild type (B, dorsal; C, lateral), Df/optix (D, laterodorsal; E, ventral) head skeletons, tsh^- optix^C01718 HoxCP (F, ventral) and nullo-Gal4 UAS-optix (G, lateral) larva. Derivatives of the labrum (red; lr, labrum; es, epistomal sclerite) and labral sense organ are absent and the maxillary-derived, mouth hooks (MH) are missing (E) or reduced (D) in Df/optix (compare B-E). All sense organs, apart from the labral one, are unaffected in the different optix alleles (D,E) and RNAi-treated larvae (not shown). In E, the asterisk indicates head cuticle in place of the mouth hooks, labral and adjacent structures normally found in this position (see B,C). H piece (H), hypostomal sclerite (hys) and lateralgräten (LG) derive from segments posterior to the labrum (Jürgens et al., 1986). The head-like cuticle present on the ventral side of tsh^- optix^C01718 HoxCP (arrowhead in F) and tsh^-HoxCP (Fig. 1C) embryos are indistinguishable. In G, early ectopic expression of optix results in a failure in retraction of the germ band.

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