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Figure 1


Fig. 1. Le-Cre is specifically expressed in lacrimal gland. (A-C) The Le-Cre transgene GFP expression. GFP expression from the Le-Cre transgene was first visible in the E14.5 lacrimal gland bud (arrowhead), and it continued to be present in the elongated lacrimal gland (arrowhead) at E16.5 and lacrimal gland lobes (arrow) at P0. (D-F) Co-expression of Pax6, GFP and Cre in the Le-Cre embryo. In contrast to the nuclear Pax6 expression in retina and lacrimal gland (D), cytoplasmic GFP and nuclear Cre were found exclusively in the lacrimal gland bud (E,F). (G-I) The Le-Cre transgene Cre activity. Cre recombinase activity in the Le-Cre transgene was determined by crossing with R26R mice, which marked all the Cre-expressing cells and their progenies with lacZ expression identifiable by X-gal staining (G). In P0 lacrimal gland sections, all X-gal-stained cells resided in the Pax6 positive epithelium, demonstrating the restricted expression of the Le-Cre transgene.





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