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Fig. 2. Hr39 expression. (A) Hr39 expression levels in mutants using RT-PCR. As an internal control, RpS17 was amplified. (B) Comparison of Hr39 expression among strains using real-time quantitative RT-PCR. Standard deviation of each triplicate (±s.d.) is shown. (C-E,L-N) Green indicates Hr39-lacZ detected via anti-β-gal antibody. Magenta indicates DNA. (F-K) Hr39 in situ hybridization (green) (C) Larval ring gland, Hr39⁰³⁵⁰⁸/+. Blue indicates anti-Fas2. Scale bar: 75 µm. (D) Female genital disc, Hr39⁰³⁵⁰⁸/+. Scale bar: 50 µm. (E) Female larval gonad, Hr39⁰⁷¹⁵⁴/+. Scale bar: 50 µm. Wild type (F-J); Hr39⁰⁴⁴⁴³ (K,L). (F) Reproductive tract; (G) parovaria; (H) seminal receptacle; (I) spermathecae-associated fat body; (J-L) spermathecae. (M) Hr39⁰³⁵⁰⁸/+ ovariole. Scale bar: 100 µm. (N) Hr39⁰³⁵⁰⁸/+ testis. Scale bar: 100 µm. SR, seminal receptacle; SP, spermatheca; P, parovarium; O, oviduct; FB, fat body.

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