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Fig. 4. Spatial and temporal correlation between the wild-type patterns of Dpp
signaling and Br expression. (A-C) P-Mad (green) and Br expression
(red) at different stages of oogenesis; see text for details. (D-G) The
spatial pattern of br transcript at different stages of oogenesis.
Stage 9, low level of expression throughout the follicular epithelium (D);
stage 10A, repression in the anterior and dorsal midline follicle cells (E);
stage 10B, expression in the roof cells (F); stage 11 and onward, no
expression throughout the follicular epithelium (G). (H-J) A mosaic egg
chamber with a large Medea- (Med-)
clone that spans the lateral and anterior regions of the follicular
epithelium; Med- cells are marked by the absence of GFP
(H, green). The clone generates ectopic Br expression in the anterior cells,
but does not disrupt the lateral expression of Br (I). (J) Enlarged view of Br
expression in the boxed area in H. (K-M) A clone of
Mad- cells, which spans both the roof cell domain and the
dorsal midline, does not affect Br levels in the dorsal midline cells and does
not disrupt Br expression in the roof cells. Br expression is shown in red;
Mad- cells are marked by the absence of GFP (green). A
similar effect can be seen with a lateral of clone Med-
cells (N-P). Midline levels of Br are also not affected by removal of
Med in the dorsal midline (not shown). In all images the dorsal
midline is marked by an arrowhead, anterior is to the left, and the clones are
outlined by a dotted line.
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