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Fig. 7. Br controls Dpp signaling in the follicle cells by regulating the
expression of tkv. (A-D) Uniform expression of Br-Z1
isoform in the follicular epithelium leads to ectopic expression of
tkv and Dpp signaling. (A-C) Br (A) and P-Mad (B) patterns in a stage
12 egg chamber overexpressing Br-Z1. P-Mad signal is detected in the midline
and anterior follicle cells, where it is absent in the wild-type egg chamber
at this stage. The image in C is a merge of Br (red) and P-Mad (green)
patterns. (D) Overexpression of the Br-Z1 isoform generates ectopic expression
of tkv: tkv is expressed in the dorsal midline (denoted by
the arrowhead), where it is repressed in the wild type (see also Fig. S3 in
the supplementary material). (E-K) Clones of br-
cells (marked by an arrow) generate defects in the spatial patterns of Dpp
signaling and tkv expression. The arrowhead marks the dorsal midline.
(E-J) P-Mad pattern (red) in mosaic epithelial layers with the GFP-marked
clones of br- cells. (K) Defective pattern of tkv
(denoted by the arrow) in the egg chamber with a clone of
br- cells. Dorsal view; the arrowhead marks the midline.
(L) Model summarizing the results of experiments in A-K: Br regulates
tkv, which is essential for Dpp signaling. (M) ESEM image of
the eggshell morphology induced by the overexpression of the Br-Z1
isoform.
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