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Fig. 3. Patterning defects caused by overexpression of fgfA in sea
urchin. Embryos overexpressing fgfA or treated with bFGF were
observed at the early gastrula stage (A,B) or at 48 hours
(C-H) or fixed at the mesenchyme blastula stage for in situ
hybridization with a pax2/5/8 probe (I-L). fgfA
overexpression induces radial (K) or broadened (L) expression of
pax2/5/8. See text for details. vv, vegetal view. Arrows indicate the
positions of the spicule rudiments; red square indicates the presence of an
ectopic spicule.