spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Fz function is required for Wnt-induced LRP6 phosphorylation. (A) Shisa inhibited Wnt3a-induced LRP6 phosphorylation. HEK293T cells cotransfected with LRP6 plus Flag-tagged Shisa (or a control vector) were incubated with Wnt3a conditioned medium (CM) or the control CM for 1 hour. Shisa inhibited Wnt3a-induced β-catenin accumulation in the cytosol. β-actin: a loading control. (B) L cells stably transfected with shRNAs against Fz2 and Fz7 [L (Fz-) cell] showed diminished Wnt-induced LRP6 (endogenous) phosphorylation. The L (Fz-) cells were treated for 1hour with increasing concentrations of Wnt3a CM. These L (Fz-) cells also exhibited attenuated β-catenin stabilization in response to Wnt3a compared to the control L cells. (C) Human Fz5 expression rescued Wnt3a-induced LRP6 (endogenous) phosphorylation in the L (Fz-) cells. The stable clones of L (Fz-) cell transfected with Fz5 (or the control vector) were pooled together and assayed.





Right arrow Return to article