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Fig. 5. sprouty gene expression and tissue-specific inactivation in the
developing mouse incisor. Gene expression was analyzed by RNA in situ
hybridization using the probes indicated on paraffin sections of embryonic
incisors of the genotypes denoted at E16.5 or 17.5. (A-F) A comparison
of the expression domains of sprouty gene family members in the incisor at the
stages indicated. Yellow asterisks indicate mesenchymal expression.
(G-K) Tissue-specific inactivation of Spry4. The absence of
Spry4 expression is indicated by red circles in the epithelium of an
incisor carrying K14-cre, one Spry4fl and one
Spry4- allele (G) and by red asterisks in the mesenchyme
of an incisor carrying Wnt1-cre and two Spry4fl
alleles (H). For each genotype shown in I-K, the diagram illustrates the
tissue in which Cre-mediated recombination occurred (green fill), the sprouty
alleles that were inactivated by Cre (white lettering), or that were inherited
as nulls (black lettering). The photograph shows Shh expression,
which marks cells that are differentiating along the ameloblast lineage. The
red arrowheads point to the ectopic lingual Shh expression
domain.
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