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Fig. 5. lbk larvae show a compromised ability to clear bacterial
infections. (A,B) Dorsal views of larvae in which
macrophages were stained with Neutral Red to reveal intracellular vesicles
(arrows). Compared with the vesicles observed in wild-type macrophages, those
in lbk appear enlarged, heterogeneous in size and less regularly
shaped. Insets show higher magnifications of macrophage vesicles. (C)
Combined bright-field and fluorescence images of the tail of a 48 hpf larva 5
minutes after the injection of DsRed-fluorescent bacteria, showing bacteria
still circulating in the bloodstream (inset; fluorescent traces of bacteria
moving in blood vessels) and bacteria that have been engulfed by phagocytes
(arrowheads). (D-G) Head regions of sibling and lbk larvae 32
hours after bacteria injection. Fluorescent bacteria-laden phagocytes are
present in lbk (arrowheads). (H,I) Bright-field (H) and
fluorescence images (I) of a bacterial plate showing bacterial colonies from
an lbk larva macerated at 85 hpf, 37 hours after Salmonella
injection. (J-M) Tails of sibling and lbk larvae 32 hours
after bacteria injection. Arrowheads indicate fluorescent bacteria-containing
phagocytes.
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