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Fig. 6. AML1-ETO reprograms hematopoietic cell fate, converting erythropoiesis
to granulopoiesis. (A) In situ hybridization of gata1,
fluorescent images of zpu.1-EGFP transgenic fish, and in situ
hybridization of mpo and l-plastin. AML1-ETO expression
results in gata1 downregulation. Subsequently, pu.1
expression is increased. Finally, the accumulated blood cells express the
granulocytic cell marker mpo but not the monocytic cell marker
l-plastin. All embryos were subjected to the heat treatment and then
were collected at designated stages as indicated. (B) Proposed effects
of AML1-ETO in hematopoietic progenitor cells. MPC, myeloerythoid progenitor
cell; GMP, granulocyte/monocyte progenitor; MEP, megakaryocyte/erythroid
progenitor. The red crosses indicate the steps suppressed by AML1-ETO. The
parentheses indicate the markers for each cell type or the genes involved in
the processes. These data suggest that AML1-ETO reprograms hematopoietic cell
fate, resulting in an enrichment of myeloblasts that express mpo
(boxed and shaded).
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