DEV019760 Supplementary Material

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• Supplemental Figure S1 (Adobe PDF) -

  Fig. S1. Inhibition of RhoA interferes with the Sema4d-induced inhibition of ureteric branching morphogenesis. (A-D) Typical examples of anti-calbindin-stained metanephroi cultured with either Sema4d or mock medium in the presence or absence of the membrane-permeable RhoA inhibitor TAT-C3 (0.5 µM). (E-H) In the presence of 0.5 µM TAT-C3, the inhibitory effects of Sema4d on ureteric branching (E,F) and kidney size (G,H) are reversed, and both parameters are increased over control levels. *P<0.05 when compared with mock treated or Sema4D alone; Student's paired t-test.

• Supplemental Table S1 - Adobe PDF