spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Effects of blockade of endogenous Sema4d on metanephroic development. (A) Western blot analysis demonstrating the efficacy of a Sema4d function-blocking antibody (anti-Sema4d), but not preimmune serum (pre.; control), in attenuating Sema4d-induced RhoA activation in a GST-RBD-based assay in HEK293T cells transfected with plexin B1 (plxnB1) and PDZRhoGEF (PRG). Anti-Myc-positive bands in western blotting serve as loading controls. (B,C) Typical examples of the anti-calbindin-immunoreactive ureteric tree (green) in sister metanephroi from E12 embryos cultured in the presence of an anti-Sema4d antibody (C) or preimmune serum (B, control). (D,E) E12 metanephroi cultured with the anti-Sema4d antibody showed significantly more branching (D), but were the same size (E), when compared with metanephroi cultured with preimmune serum (control). *P<0.05, Student's paired t-test. Scale bars: 250 µm.





Right arrow Return to article