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Fig. 7. Role of PGPs and PINs in the regulation of the spatial distribution of
the auxin response. (A-H) Roles of PGP1/PGP19 and PIN1 in auxin
response distribution (as visualized by DR5rev::GFP) in heart-stage
embryos (A-D) and root tips (E-H). Wild type (A,E). Increased signal in
pgp1pgp19 (B,F), decreased signal in pin1 (C,G) and
pronounced defects in the distribution of the DR5 signal in the
pin1pgp1pgp19 embryos (D) are seen, but restoration occurs in roots
(H). At least five roots or embryos from all mutant combination were
simultaneously analysed in two independent experiments (for pin1 and
pin1pgp1pgp19 mutants, only embryos with visible phenotypes were
analysed). Arrowheads in A-D indicate auxin maxima in cotyledon primordia.
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