|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 11. Elimination of endoderm development in osr1 morphants rescues
proximal pronephric phenotype. (A-D) Expression of pax2a
in control (A), osr1 morphant (B), sox32 morphant (C) and
osr1+sox32 double morphants (D). Loss of osr1
resulted in loss of proximal pax2a pronephric expression (arrowhead,
B) when compared with control 18-somite stage embryos (arrowhead, A).
sox32 (cas) morphants lacking endoderm showed slightly
enhanced expression of pax2a in the proximal nephron (arrowhead, C).
The loss of pax2a expression in the proximal pronephros of
osr1 morphants was rescued by elimination of endoderm in the
osr1+ sox32 double morphants (arrowhead, D). (E-G)
Expression of pax2a in uninjected bon heterozygote incross
embryos (E) and osr1 morpholino injected bon incross embryos
(F,G) at 24 hpf. pax2a expression was lost from the proximal
pronephros in 47 out of 64 of the injected embryos (73%) (F). Loss of
pax2a expression from the proximal pronephros was rescued in 17 out
of 64 (27%) osr1 MO injected, bon heterozygote incross
embryos. Arrowheads in E-G indicate expression of pax2a in the
proximal pronephros.
|
