DEV024612 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF

• Supplemental Table S2 - Adobe PDF

• Supplemental Table S3 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Math5^Neθ^{rod1-KI/Math3-KI} retinas show a slight increase in photoreceptor cells but no differences in amacrine cells, bipolar cells and Müller glial cells. (A-F) Photoreceptor cells were detected with an anti-opsin antibody (A,B), and amacrine cells with anti-ChAT and anti-p57Kip2 (C,D), or anti-calretinin and anti-calbindin antibodies (E,F). (G-J) Bipolar cells were detected with anti-Chx10 antibody (G,H), and Müller glial cells with anti-Sox9 antibody (I,J). The arrowheads in A and B indicate the photoreceptor outer segment. The density of outer segments in Math5^{Neurod1-KI/Math3-KI} retinas is higher than that in wild-type retinas. Arrowheads in E,F indicate horizontal cells, where calbindin is highly expressed. Weaker calbindin-positive amacrine cells are interspersed with the calretinin-positive amacrine cells below the horizontal cells.