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Fig. 2. Dkk1 selectively affects DA cell number, but not neighboring basal
diencephalic neurons. (A-F) Lateral views of either WT zebrafish
embryos (A,C,E) or their dkk1 mRNA-injected siblings (B,D,F). Embryos
were fixed at 48 hpf and subjected to whole-mount in situ hybridization with
RNA probes directed against the hypothalamic neuropeptide isotocin (zebrafish
ortholog of oxytocin) (it; A,B), hypocretin (hcrt; C,D) or
somatostatin (ss; E,F). All specimens were then subjected to
immunostaining with an anti-tyrosine hydroxylase (TH) antibody to detect DA
neurons. (G) Schematic representation of the examined diencephalic cell
types in a 48-hpf WT embryo. The positions of DA group 2 (Gr. 2) and groups
3-6 (Gr. 3 to 6) are indicated. (H) Bar chart presenting average counts
of dat-expressing DA group 2 cells (DA), isotocin (IT),
hypocretin/orexin (Hcrt) or somatostatin (SS) expressing cells in WT and
dkk1-injected embryos at 48 hpf. The number of embryos analyzed (n)
is shown below. Dien, diencephalon; LC, locus coeruleus; Po, preoptic area;
Tel, telencephalon. Scale bar: 50 µm.
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