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Fig. 4. Specific neurog1+ precursor pools differentially
respond to Wnt signaling. (A,B) Projected confocal
z-stack images (lateral view) of DA (red) and
neurog1+ (green) cells in neurog1::gfp transgenic
zebrafish reporter embryos. Embryos were injected with either a vehicle
solution (A) or an mRNA encoding Dkk1 (B). (A',B')
Bubble-plot graphic representation of the x, y, z cell position of
all neurog1+ (green) and DA (TH+
neurog1+) cells (red) in the PT. Bubble size was derived
from the position of the cells along the z-axis to give the
impression of depth. (C) Schematic depicting the major diencephalic DA
cell groups in the developing embryo at 48 hpf, relative to the
neurog1::gfp expression domains. The area indicated by the dashed box
corresponds to the posterior tuberculum domain as shown at high-magnification
in A,B. (D) Bar charts showing the averaged number of
neurog1+ and DA (TH+
neurog1+) cells in embryos that were injected with either
a vehicle solution or dkk1 mRNA (n=5). Hyp, hypothalamus;
NPO, neurosecretory preoptic nucleus; PT, posterior tuberculum; Tel,
telencephalon. Scale bar: 25 µm.
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