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Fig. 8. Canonical Wnt pathway components restrict DA cell number.
(A-E) Lateral views of zebrafish embryos that were injected with
vehicle solution (WT, n=71; A), wnt8b morpholino (MO)
(n=20; B), fz8a MO (n=11; C) or lef1 MO
(n=18; D) and of the lef1-deficient X8 deletion
mutant (n=18; E). Embryos were fixed at 24 hpf and subjected to in
situ hybridization with a dat probe. The result of the double
knockdown of wnt8b and fz8a (n=44) is presented in
F. (F) Bar chart summarizing average cell counts of DA group 2 neurons
in the genetic backgrounds shown in A-E. (G,H) Lateral views
(anterior to the left) of embryos that were injected with vehicle solution (G)
or wnt8b MO (H). Embryos were fixed at 24 hpf and subjected to
whole-mount in situ hybridization with an RNA probe directed against
barhl2. Scale bar: 50 µm.
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