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Fig. 4. Downregulation of Fgf3 and Fgf19 expression
downregulates both early and late otic genes. (A) The introduction
of shFgf3 downregulates endogenous expression of Fgf3 in the
mesoderm of 1-4ss chick embryos. Sections show a reduction of Fgf3
expression in regions that express the mCherry tracer. (B) Introduction
of shFgf19 reduces endogenous Fgf19 expression in the
mesoderm of 1-4ss chick embryos. Sections show a reduction of Fgf19
expression in regions that express the mCherry tracer. (C) Control
embryos electroporated with control shScrambled show no change in
Pax2 expression (n=19/21). (D)shFgf3
electroporation results in a slight reduction of Pax2 OEPD expression
(n=17/38). (E)shFgf19 causes a slight reduction in
Pax2 expression (n=15/30). (F) Electroporation of
both shFgf3 and shFgf19 causes a strong reduction of the
normal Pax2 OEPD expression domain (n=13/21). (G)
Control embryos electroporated with shScrambled show no change in
Soho1 expression (only one out of 10 showed aberrant expression).
(H)shFgf3 electroporation results in a slight reduction of
Soho1 expression (n=8/14). (I)shFgf19 causes
a slight reduction in Soho1 expression (n=9/15). (J)
Electroporation of both shFgf3 and shFgf19 causes a strong
reduction of normal Soho1 otic expression (n=12/19). In all
cases, the electroporated side is on the right.
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