DEV023986 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. The GW and the NB2-4t motoneurons undergo apoptosis and are removed from the CNS. (A-B′) TUNEL staining (green) and Eg staining (red) at late stage 16 in single scans of wild-type embryos (A′ and B′ show only the Eg staining.). (A) GW (outlined) shows TUNEL staining in segment A7. (B) An MNa (outlined) in segment T3 is TUNEL positive. (C) GFP staining in the L1 brain of an eg>GFP^nuclear embryo. An NB7-3 lineage is enclosed by the dashed circle. Arrowheads point to the surviving GWs in segments T1 and T2. GWs are outlined in segments where they lie beneath or above an interneuron. NB7-3 comprises four neurons in segments T1 and T2, but only the three interneurons remain in T3 and the abdominal segments. Dashed lines mark the midline; anterior is up in all images.

• Supplemental Figure S2 (Adobe PDF) -

  Fig. S2. Expression of Ubx in NB7-3 and NB2-4 in different developmental stages. Ubx expression is shown in the early neuroblasts at stage 11 for NB7-3 (A-C) and NB2-4 (J-L). At stages 14 (D-F,M-O) and 15 (G-I,P-R), all visible NB7-3 progeny are outlined and GWs are indicated by arrows in D-I, and only the NB2-4 motoneurons are outlined and the MNas indicated by arrows in M-R. Dashed lines mark the midline. (A′-R′) Only the Ubx staining is shown. All images are single scans of given lineages. Anterior is up in all images.