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Figure 9


Fig. 9. Differentiation of mesDA neurons requires Otx2. (A-P'') Adjacent sections through the intermediate mesencephalon of E11.5 and E12.5 wild type, En1Cre/+; tOtx2ov/ov and En1Cre/+; Otx2flox/flox embryos immunostained with Nkx6.1 and Nkx2.2 (A-B''), Mash1 and Sox2 (G-H''), Ngn2 and Sox2 (I-J''), Nurr1 and Ngn2 (K-L''), Nurr1 and Pitx3 (M-N''), Lmx1b and Otx2 (O,O''), Lmx1b and 5-HT (P,P'') or hybridized with Lmx1a (C-D'') or Msx1 (E-F'') probes. In En1Cre/+; Otx2flox/flox mutants, Lmx1b is dorsally expanded within the Nkx2.2+ domain that generates 5-HT+ neurons, and is abundant at E12.5 in the Sox2+ mesDA progenitors (O'',P''). The arrow indicates the ventral border of Nkx6.1+ domain in wild-type and En1Cre/+; tOtx2ov/ov embryos and the ventral border of the Nkx2.2+ domain in En1Cre/+; Otx2flox/flox mutants.





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