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Fig. 2. Molecular markers and cell position can be used to identify each RP
motoneuron in the NB3-1 lineage. (A) RP1 and RP4 are
Hb+, Kr+, Cut-, Zfh2- and are
shown in insets because they are usually obstructed in the projection
(n>100). RP3 is Hb-, Kr+, Cut-,
Zfh2+ (n>100). RP5 is Hb-, Kr-,
Cut+, Zfh2+ (n>100). A single representative
hemisegment of a wild-type (wt) stage-16 CNS is shown as a maximum intensity
projection. Midline, left; anterior, up. An expression summary is shown above.
(B) (Top row) RP motoneurons are Islet+ HB9+
(outlined). RP1 and RP4 occupy the deepest layer; RP1 is more dorsal and
expresses HB9 at higher levels than does RP4 after stage 15
(n>100). RP3 is directly ventral to RP1 and RP4
(n>100). RP5 is ventral and anterior to RP3 (n>100).
White arrowheads, NB7-3-derived EW interneurons. Dashed vertical line,
midline. Ventral views of two segments are shown from deep (left) to
superficial (right) focal planes. (Bottom row) Each RP neuron has a non-RP
neuron sibling, based the duplication of each RP neuron in sanpodo
mutants. Eight Islet+ HB9+ Late Bloomer+ RP
motoneurons are observed in each hemisegment (quantified in
Table 1). Midline, between each
pair of panels. (C) Recombination-induced activation of lacZ
in NB3-1 labeled RP4, RP3, RP5 and the late-born interneurons but not RP1,
showing that RP1 is the first-born neuron in the lineage. HB9, green;
β-galactosidase, magenta. RP neurons and NB3-1 are outlined and labeled.
One hemisegment of a stage-16 CNS is shown as a maximum intensity projection.
Midline, left; anterior, up. A schematic summary of the clone is shown to the
right. (D) Schematic of NB3-1 gene expression and cell lineage. The
vertical dashed line represents the transition between RP neuron and
subsequent interneuron specification. Nb, sibling cell fate specified by Numb;
N, sibling cell fate requires Sanpodo and active Notch signaling. Scale bars:
3 µm.
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