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Fig. 5. Expression of diverse genes involved in endosperm and embryo development
in zou mutant seeds. (A-D) Images of in situ hybridization of
digoxigenin-labelled ALE1 antisense RNA probes to sections of
siliques, viewed using DIC microscopy. Embryo is indicated by arrowhead,
endosperm by arrows. Little or no ALE1 expression was detected in
zou-2 mutant seeds (C,D), whereas wild type showed intense signal in
ESR (A,B). (E,F) Confocal images of reporter line 9185 in
wild-type (E) and zou-4 (F) seeds. (G,H) Images of
reporter line ATML1::GFP-ATML1 in wild-type (G) and zou-4
(H) seeds. (I,J) Images of reporter line ACR4::H2B-YFP
in wild-type (I) and zou-4 (J) seeds. Scale bar: 100 µm.
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