DEV022319 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Contribution of ENCCs to the development of each region of the mouse gastrointestinal tract. ENCCs were visualized by β-galactosidase activity at E12.5 in control/R26R (A-D) and Pofut1cKO/R26R (E-H) embryos. The regions of the gastrointestinal tract analyzed are indicated above the top panels.

• Supplemental Figure S2 -

  Fig. S2. Proliferative ENCCs are localized in the cecum. Immunohistological analysis of control/CAG-CAT-EGFP and Pofut1 cKO/CAG-CAT-EGFP embryos at E12.5 using antibodies against BrdU (red) and GFP (green). Open arrowheads indicate proliferative ENCCs. The specific regions of the gut analyzed are indicated on the left.

• Supplemental Figure S3 -

  Fig. S3. Ednrb expression in Pofut1 cKO embryos. (A-L) Whole-mount in situ hybridizations were performed using RNA probes against Ednrb (purple) in control and Pofut1 cKO embryos at E11.5 and E12.5 as labeled. The gastrointestinal tracts are outlined (dotted lines). (L) An enlarged image of the cecum indicated by the dashed box.

• Supplemental Figure S4 -

  Fig. S4. Expression of Notch signaling genes in the gastrointestinal tract. The same images as in Fig. 6 are presented with a single channel to detect in situ signals (magenta). Neural crest cells are outlined (dotted white lines). Open arrowheads indicate expression in ENCCs, and solid arrowheads indicate expression in neighboring cells.

• Supplemental Figure S5 -

  Fig. S5. Sox10 expression in Dll1-null mutant embryos. Immunohistological detection of Sox10 (red) expression within ENCCs (p75^NTR, green) in control (A-D) and Dll1-null (E-H) embryos at E11.5.