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Fig. 3. Detection of apoptosis and proliferation in ENCCs. (A-H)
Immunohistological analysis of control (A-D) and Pofut1 cKO (E-H)
mouse embryos using anti-caspase 3 (red) and anti-GFP (green) antibodies to
visualize apoptotic cells and ENCCs, respectively, at E11.5 (A,B,E,F) and
E12.5 (C,D,G,H). (I-P) Immunohistological analysis of control (I-L) and
Pofut1 cKO (M-P) embryos using anti-BrdU (red) and anti-GFP (green)
antibodies to visualize proliferating cells and ENCCs, respectively, at E11.5
(I,J,,M,N) and E12 (K,L,O,P). Arrowheads indicate proliferating ENCCs.
(Q,R) Quantitation of ENCC proliferation at E11.5 (Q) and E12.5
(R). Average percentages of BrdU-incorporating ENCCs are shown with s.d. ns,
not significant.
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