QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Expression of Fz5 in the embryonic eye field and adult retina. (A,B) Whole-mount Fz5^CKO-AP/+;Sox2-Cre mouse embryos stained with NBT/BCIP and viewed from the front. At E8.5, Fz5 is expressed in the anterior neural plate (NP). At E9.5, Fz5 expression is predominantly localized to the optic vesicles (OV). In B, the open cephalic neural tube can be seen above the zone of hybridization. (C,D) X-Gal-stained Fz5^lacZ/+ embryo at E12.5 showing Fz5 expression in the ventral telencephalon (TE), eye and optic nerve (ON). D shows a vibratome section, the location of which is indicated by the line in C. (E,F) NBT/BCIP-stained flat-mount of a Fz5^CKO-AP/+;R26-CreER adult retina. Sparse labeling was achieved by low-dose 4-hydroxytamoxifen injection (see Materials and methods). The boxed region in E is shown enlarged in F. Fz5 expression is restricted to Müller glia (left arrowhead) and to a subset of amacrine cells (right arrowhead). (G) A vertical section through the retina in E showing an AP-labeled Müller glial cell (left) and an amacrine cell (right). Here, and in all other retinal cross-sections, the outer nuclear layer is at the top of the image and the ganglion cell layer is at the bottom.

Return to article