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Fig. 4. Excess mesenchymal cells in the prenatal vitreous cavity and persistent
fetal vasculature in the postnatal Fz5-/- eye.
(A,B) DAPI-stained (A) WT and (B) Fz5-/-
mouse embryonic eyes at E14.5. Arrowhead in B indicates the excess cells
between the lens and retina. (C,D) Anti-smooth muscle actin
(SMA) immunostaining of whole-mount retinas at P5. The hyaloid vessels and
developing retinal vasculature emerge from the optic disk (green arrowheads).
The cell mass that is attached to the optic disc in the
Fz5-/- retina contains smooth muscle actin (D, red
arrowhead). (E-H) Intact retinas viewed from the vitreal face (E,F) and
Hematoxylin and Eosin-stained sections through the retina and optic nerve
(G,H) at 
P56. The ectopic tissue (red arrowhead in F) is attached at the
optic disc of the Fz5-/- retina (green arrowhead in F) and
contains numerous pigment cells. (I-L) Semi-thin, plastic-embedded
sections through the central retina showing the vascular structure and
connectivity of the intra-vitreal tissue at P56. The serial sections at
low magnification (I,J) show ectopic tissue attached to the optic disc
(bottom) and the posterior face of the lens (top; green arrowheads). The large
blood vessels in I and J are contiguous (red arrowheads). (K)
High-magnification view of the boxed region in I, showing an intermingling of
pigmented and unpigmented cells surrounding a large blood vessel (asterisk).
(L) At the edge of the optic disc, pigmented cells associated with the initial
segment of the intravitreal vasculature (asterisk) are contiguous with the
pigmented cells of the choroid (arrowheads).
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