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Fig. 8. Comparison of Pax2 immunolocalization during and after optic fissure
closure in WT versus Fz5-/- retinas. In A-L, pairs of
nearby sections are shown in order to better illustrate the staining and
visualize the three-dimensional anatomy. (A-D) Anti-Pax2 immunostaining
at E13.5 showing the posterior expansion of the domain of Pax2 expression in
the Fz5-/- optic fissure. Arrowheads indicate the
posterior border of the optic fissure and the zone of Pax2 expression that
tracks the optic fissure. (E-H) At E14.5, when Pax2 is no longer
detected in the ventral retina in the WT mouse, it continues to mark the
ventral fissure in the Fz5-/- retina. A-H are sectioned in
the horizontal plane; nasal is upward, as indicated in A (N, nasal; T,
temporal). (I-L) At E14.5, Pax2 abundance in the optic disc and optic
nerve is similar in WT and Fz5-/- retinas.
(M,N) Whole-mount immunostaining at E16.5 showing Pax2 in the
ventral fissure region of the Fz5-/- retina. Insets show a
plane of focus within the optic disc, showing a ring of Pax2-positive cells
around the optic nerve. (O,P) The number and lateral
distribution of Pax2-expressing astrocyte precursors at the vitreal face of
the retina are increased in the Fz5-/- eye.
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