DEV022350 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. The Wnt1 promoter and the Pax3 1.6 kb proximal promoter drive Cre expression in neural crest cells with similar kinetics. (A-F) Whole-mount lacZ staining (blue) on E8.5 embryos of the indicated somite (som) number and genotype. The Rosa26R locus has been engineered (R26R^lacZ) to express lacZ in cells where Cre is active. Both the proximal Pax3 promoter-driven Cre (Pax3Cre) and Wnt1-driven Cre (Wnt1Cre) are active in rhombomeres R2, R4 and R6 and lineage-mark neural crest cells beginning around the 10-somite stage. Wnt1Cre is active in additional neural crest populations, driving lacZ expression in neural crest cells migrating to the first branchial arch (BA1). C and F are dorsal views of the embryos shown in B and E and demonstrate that both Wnt1Cre and Pax3Cre expression initiates in R6, which provides cardiac neural crest cells, around the 16-somite stage. OV, otic vesicle.