|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Wild-type OFT myocardium rescues the endothelial-to-mesenchymal
transformation defects of Fgf8;Isl1Cre mutants. (A)
Explant (Ex) culture of mouse E9.5 control OFT. (A') Close-up of
boxed region in A. Arrowhead demarcates a rare, rounded cell that migrated
from the explant but failed to invade. (B) Explant culture of an E9.5
Fgf8;Isl1Cre mutant OFT reveals failure of endothelial EMT.
(B') Close-up of boxed region in B. Arrows point to rare cells
that underwent EMT. (C,C') Co-culture of E9.5 OFTs from
Rosa26lacZ;Isl1Cre (blue) and controls (white). (C')
Enlargement of boxed area in C. (D,D') Co-culture of
control and Fgf8;Rosa26lacZ;Isl1Cre E9.5 OFTs shows rescue
of mutant cells (blue, arrowheads). (D') Enlargement of boxed area in D.
(E,E') Complementary experiment to D; control cells are
β-galactosidase-positive (blue) and rescued Fgf8 mutant cells
(arrowheads) are white. (E') Enlargement of boxed area in E. For
quantitated data, see Fig. S2 in the supplementary material.
|
