spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Compromised NCC contribution to the Frs2{alpha}cn/Nkx OFT cushions. (A) Immunostaining with anti-AP2{alpha} antibody reveals reduced numbers of migrating NCCs in the aortic sac (AS) and in pharyngeal arches (PAs) 3 and 4/6 in Frs2{alpha}cn/Nkx, but not in Frs2{alpha}cn/Mef, mouse embryos at E9.5. (B) The numbers of AP2{alpha}+ cells in the aortic sac and in pharyngeal arches 3 and 4/6 as scored from five individuals (mean ±s.d.). (C) Co-immunostaining reveals that proliferation of cardiac NCCs is compromised in Frs2{alpha}cn/Nkx embryos. Proliferating cells are labeled with anti-phosphorylated histone H3 antibody (red), NCCs with anti-AP2{alpha} antibody (green) and nuclei with To-Pro3 (blue). Triple-positive cells are indicated by arrows. (D) Statistical analyses of proliferating cardiac NCCs from five individuals (mean ±s.d.).





Right arrow Return to article