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Fig. 1. Rspo3 is expressed in endothelial cells and their
precursors. (A-K) Rspo3 expression by in situ hybridization
during mouse and Xenopus embryogenesis. (A-D) Mouse embryo. (E-H)
X. laevis. (I-K) X. tropicalis. (A) E8.0 embryo;
anterior towards the left. Rspo3 expression is in the primitive
streak and in the allantois. (B) E8.5 embryo; anterior towards the top.
Rspo3 expression is prominent in the posterior mesoderm and in the
allantois. (C) Rspo3 expression in vasculature of the
chorionic plate (E8.5). (D) Expression in the umbilical vein
(arrowhead) (E12.5). (E) Stage 17; dorsal view, anterior leftwards,
showing expression in the neural plate. (F) Stage 28; lateral view with
anterior leftwards, showing expression in organs labeled. (G,H)
Transverse section at tailbud stage showing expression of Rspo3 (G)
and Vegf (H) in the hypochord (arrowheads). (I-K) Expression
of indicated genes in stage 24; ventral view, anterior leftwards, showing
expression of Msr and Rspo3 in the periphery of the ventral
blood islands. Abbreviations: all, allantois; ba, branchial arches; cp,
chorionic plate; fb, forebrain; dnt, dorsal neural tube; dlp, dorsal lateral
plate; lb, limb bud; n, notochord; pm, posterior mesoderm; pn, pronephric
sinus; ps, primitive streak; tb, tailbud mesoderm; vbi, ventral blood
island.
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