spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Rspo3 is required for Wnt/β-catenin mediated induction of Vegf. (A) Vegfr2 expression. (B) Vegfa expression. (C) Tpbpa expression in spongiotrophoblast layer. (D) Csh1 (placental lactogen 1) expression in giant cell border. (A-D) Representative samples from 20 wild-type and 20 knockout embryos are shown as indicated. (E) Immunofluorescence staining of β-catenin (green) and nuclei (Hoechst, blue) in the allantois (E8.5). Reduced β-catenin staining occurs in Rspo3-/- allantois. (F) BATGAL reporter activity in wild-type and Rspo3-/- chorionic plates. Representative samples from 20 wild-type and 20 knockout embryos are shown. (G) Quantification of lacZ staining in the allantois (all) (E8.5), somites (s) (E8.5) and chorionic plate (cp) (E9.5) and β-catenin staining in the allantois (all) (E8.5). The number of positive cells in wild-type embryos was set to 100% (see Materials and methods). Abbreviations: all, allantois; ch, chorion; gc, giant cells; sp, spongiotrophoblast; la, labyrinth; cp, chorionic plate.





Right arrow Return to article