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Figure 3


Fig. 3. Kymograph analysis of GFP:MEX-5. (A) Kymograph display of images collected from live recording of a one-cell embryo; line widths are 2 microns. Imaging began before the appearance of the maternal and paternal pronuclei (mpn and ppn), when MEX-5 is symmetrical, and ended at pronuclear meeting (pnm), when MEX-5 is asymmetrical. Arrows indicate examples of GFP:MEX-5-containing particles. (B,C) One-cell embryo as GFP:MEX-5 asymmetry is initiated (decondensation) before (B) and after (C) photobleaching. (D) Kymograph of recovery within the boxed zone indicated in C; the asterisk indicates the time point prior to bleaching and line widths are 8.3 microns. (E,F) Cartoons of idealized kymographs from hypothetical embryos after photobleaching. Scale bars: in A, 4 minutes; in D, 4 seconds.





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