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Figure 4


Fig. 4. FRAP and FLIP analysis of GFP:MEX-5 mobility. (A) FRAP results on one-cell embryos with GFP or the fusion protein listed (n=4-10 embryos analyzed per experiment). P-values are from comparisons to pJT02 after pronuclear meeting (pnm); error bars show s.e.m. (B,C) FLIP experiments on embryos at pronuclear meeting. Embryos expressing GFP, NMY-2:GFP, or the GFP:MEX-5 fusion protein listed were photobleached near the posterior pole (asterisk) for the number of cycles indicated. (C) Fluorescence measured at the anterior pole (solid line) and posterior pole (dashed line) for single embryos after each photobleaching cycle for GFP or the GFP:MEX-5 fusion proteins listed. Loss of posterior fluorescence is less for GFP and GFP:MEX-5 [-ZFs; pJT132] than for the other proteins, presumably because a larger fraction of non-bleached, anterior protein moves posteriorly.





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