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Fig. 7. Pre-treatment with 100 µM spermine NONOate potentiates
responses of sperm to 3 µM progesterone. (A) When sperm
were exposed to 3 µM progesterone after pre-treatment with spermine NONOate
(100 µM for 10 minutes), the initial [Ca2+]i
transient was enlarged (in some cells) and significantly prolonged compared
with that seen in control cells (inset shows three single cell responses,
scales as for main plot). Responses of eight cells are shown. (B)
Co-stimulation with spermine NONOate increases the proportion of cells in
which a prolonged [Ca2+]i transient occurs in response
to stimulation with 3 µM progesterone. Data are plotted as a percentage of
cells in each class (defined by [Ca2+]i transient
duration). Control cells (black bars; n=27) were from the same sample
as cells exposed to NO before and during progesterone stimulation (grey bars;
n=69) and cells in which NO was washed off as progesterone was
applied (white bars; 44 cells). (C) Progesterone (3 µM) causes a
brief increase in flagellar displacement. Red line and shading show the
mean±2s.d. of frame-to-frame midpiece displacement during the control
period. Graph shows the response of one cell (representative of over 150 cells
in two experiments). (D) Pre-treatment with spermine NONOate (100
µM) prolonged and intensified the effect of progesterone on flagellar
activity. Red line and shading show the mean±2s.d. of frame-to-frame
midpiece displacement during the control period. The graph shows response of
one sperm cell (representative of over 100 cells in two experiments).
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