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Figure 1


Fig. 1. endos00003 oocytes fail to undergo meiotic maturation. (A) DAPI-stained egg chambers in different stages. nc, nurse cells; oo, oocyte; da, dorsal appendages. Asterisks indicate position of the oocyte nucleus. Scale bar: 100 µm. (B-F) Control oocytes in prophase I (B-D) and in metaphase I (E,F). Arrows indicate non-exchange fourth chromosomes. (G-K) endos00003 oocytes have a prolonged prophase I (G-J) and abnormal DNA morphology at stage 14 (K). (L-P) twine1 oocytes show similar phenotypes to endos00003 oocytes. (Q-U) elgi1 oocytes in prophase I (Q,R) and in premature metaphase I (S-U). Scale bar: 5 µm. Control (F') and elgi1 (U') have dehydrated stage 14 oocytes. endos00003 (K') and twine1 (P') oocytes are not dehydrated and have abnormal yolk morphology. Scale bar: 100 µm. (V) Quantification of DNA morphology. The number of oocytes analyzed is shown above the bars. *P<0.001.





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